cas  (Chem Impex International)

Journal: Genes to Cells

Article Title: Establishment of a Novel Caco‐2‐Based Cell Culture System for Human Sapovirus Propagation

doi: 10.1111/gtc.70007

Figure Lengend Snippet: Effect of different bile acids on HuSaV replication efficiency in Caco‐2/Cas9 cells. Caco‐2/Cas9 cells were inoculated with HuSaV GI.1 (AH20)‐positive stool suspension (approximately 4 × 10 7 copies/well in 96‐well plates) and incubated at 37°C for 3 h. HuSaV RNA copy numbers in the culture supernatants were measured at 0 dpi (immediately after medium replacement) and 7 dpi with different bile acids in the culture media. Each dot represents individual data points; bars indicate the geometric mean HuSaV RNA copy numbers; error bars represent the geometric SD. This experiment was performed once with five technical replicates. GCA, Sodium glycocholate; GCDCA, sodium glycochenodeoxycholate.

Article Snippet: In accordance with a previous study, 1000 μM sodium glycocholate (GCA; Nacalai Tesque, Kyoto, Japan) was added in the infection assay (Takagi et al. ).

Techniques: Suspension, Incubation

Journal: Genes to Cells

Article Title: Establishment of a Novel Caco‐2‐Based Cell Culture System for Human Sapovirus Propagation

doi: 10.1111/gtc.70007

Figure Lengend Snippet: Effect of different bile acids on HuSaV replication efficiency in Caco‐2MC cells. Caco‐2MC cells were inoculated with HuSaV GI.1 (M13‐18) stock at an MOI of 0.01 and incubated at 37°C for 3 h. HuSaV RNA copy numbers in the cell lysates were measured at 0 dpi (after 3 h incubation) and 5 dpi with different bile acids in the culture media. Each dot represents individual data points; bars indicate the geometric mean HuSaV RNA copy numbers; error bars represent the geometric SD. This experiment was performed three times with five technical replicates. GCA, sodium glycocholate; GCDCA, sodium glycochenodeoxycholate.

Article Snippet: In accordance with a previous study, 1000 μM sodium glycocholate (GCA; Nacalai Tesque, Kyoto, Japan) was added in the infection assay (Takagi et al. ).

Techniques: Incubation